Testing Data
KIAA1549-BRAF recombinant protein
KIAA1549-BRAF Mutant (Kex16Bex9), Active
Applications
Kinase Assay
Synonyms
KIAA1549-BRAF; KIAA1549-BRAF Mutant (Kex16Bex9); Active; KIAA1549-BRAF recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1320-1749 exon16 (KIAA1549) - 381-end or exon9-18 (BRAF)
Sequence Length
12427
Applicable Applications for KIAA1549-BRAF recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for KIAA1549-BRAF recombinant protein
Description: Recombinant human fusion KIAA1549 (1320-1749 exon16) -BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0‰Mb tandem duplication in chromosome band 7q34 (1). The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers (2).
Scientific Background: KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0‰Mb tandem duplication in chromosome band 7q34 (1). The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers (2).
Product Categories/Family for KIAA1549-BRAF recombinant protein
References
1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12 (7):621-30. 2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22 (6):841-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~125 kDa
NCBI Official Full Name
Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA
NCBI Official Synonym Full Names
KIAA1549
NCBI Official Symbol
KIAA1549
NCBI Protein Information
UPF0606 protein KIAA1549
UniProt Protein Name
UPF0606 protein KIAA1549
UniProt Gene Name
KIAA1549
NCBI Description
The protein encoded by this gene belongs to the UPF0606 family. This gene has been found to be fused to the BRAF oncogene in many cases of pilocytic astrocytoma. The fusion results from 2Mb tandem duplications at 7q34. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
Uniprot Description
KIAA1549: A chromosomal aberration involving KIAA1549 is found in pilocytic astrocytoma. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation. Belongs to the UPF0606 family. 4 isoforms of the human protein are produced by alternative promoter.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: plasma membrane
Research Articles on KIAA1549-BRAF
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Product Notes
The KIAA1549-BRAF kiaa1549 (Catalog #AAA515457) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1320-1749 exon16 (KIAA1549) - 381-end or exon9-18 (BRAF). AAA Biotech's KIAA1549-BRAF can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the KIAA1549-BRAF kiaa1549 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIAA1549-BRAF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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