Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) Recombinant Protein | CYFRA21-1 recombinant protein

Recombinant Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) Protein

Cat. Num. AAA286154

• Gene Names: KRT19; K19; CK19; K1CS
• Reactivity: Human
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: Cytokeratin Fragment Antigen 21-1 (CYFRA21-1); Recombinant Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) Protein; K1C19_HUMAN; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19.; CYFRA21-1 recombinant protein

• Host: E. coli
• Reactivity: Human
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: 58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 300 mM Imidazole, pH 8.0, Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization.
• Concentration: 0.4 mg/mL (varies by lot)
• Sequence: EKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL

• Source: Human
• Usage: CYFRA21-1 Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.
• Protein Residues: with N-terminal GST
• Preparation and Storage: Store it under sterile conditions at -20°C upon receiving.; Recommend to aliquot the protein into smaller quantities for optimal storage.; Avoid repeated freeze-thaw cycles.; ; Stability: The recombinant protein is stable for up to 6 months from date of receipt at -80°C.

SDS-PAGE

Related Product Information for CYFRA21-1 recombinant protein

Cytokeratin19 Fragment Antigen 21-1(CYFRA21-1) belongs to a family of 20 related polypeptides that are constituents of the intermediate filaments of epithelial cells. Of these CK19 is the most frequently expressed cytokeratin endometrial cancer. CYFRA21-1 is a common tumor marker of lung cancer, non-small-cell cancer, small cell carcinoma and squamous cell carcinoma. For breast cancer, CYFRA21-1 may be comparable to the sensitivity and specificity of CA153. Measured jointly with any of the tumor markers as CEA, SCCA, NSE, can increase the sensitivity. Other as bladder cancer, nasopharyngeal cancer, ovarian cancer, gastrointestinal cancer, and also have certain positive rate. CYFRA21-1 in hepatitis, pancreatitis, pneumonia, benign prostatic hyperplasia may also be increased.

NCBI and Uniprot Product Information

• NCBI GI #: 24234699
• NCBI GeneID: 3880
• NCBI Accession #: NP_002267.2
• NCBI GenBank Nucleotide #: NM_002276.4
• UniProt Accession #: P08727; Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874
• Molecular Weight: Predicted MW: 62 kDa; Observed MW: 62 kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 19
• NCBI Official Synonym Full Names: keratin 19
• NCBI Official Symbol: KRT19
• NCBI Official Synonym Symbols: K19; CK19; K1CS
• NCBI Protein Information: keratin, type I cytoskeletal 19
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: costamere; plasma membrane

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle

Biological Process: response to estrogen stimulus; sarcomere organization