Human keratin 1 ELISA Kit | KRT1 elisa kit

Human Keratin, type II cytoskeletal 1, KRT1 ELISA Kit

Cat. Num. AAA909540

• Gene Names: KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• Reactivity: Human
• Synonyms: keratin 1; Human Keratin; type II cytoskeletal 1; KRT1 ELISA Kit; type II cytoskeletal 1 (KRT1) ELISA kit; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; KRT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KRT1. No significant cross-reactivity or interference between Human KRT1 and analogues was observed.
• Sequence Length: 644

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 119395750
• NCBI GeneID: 3848
• NCBI Accession #: NP_006112.3
• NCBI GenBank Nucleotide #: NM_006121.3
• UniProt Accession #: P04264; Q14720; Q6GSJ0; Q9H298; B2RA01
• Molecular Weight: 66,039 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 1
• NCBI Official Synonym Full Names: keratin 1
• NCBI Official Symbol: KRT1
• NCBI Official Synonym Symbols: K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
• NCBI Protein Information: keratin, type II cytoskeletal 1; CK-1; cytokeratin 1; cytokeratin-1; 67 kDa cytokeratin; hair alpha protein; type-II keratin Kb1; epidermolytic hyperkeratosis 1
• UniProt Protein Name: Keratin, type II cytoskeletal 1
• Protein Family: Keratin
• UniProt Gene Name: KRT1
• UniProt Synonym Gene Names: KRTA; CK-1; K1
• UniProt Entry Name: K2C1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity; structural molecule activity; carbohydrate binding

Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis

Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis