Karyopherin Alpha 1 (KPNa1) Recombinant Protein | KPNa1 recombinant protein

Recombinant Karyopherin Alpha 1 (KPNa1)

Cat. Num. AAA2010415

• Gene Names: KPNA1; RCH2; SRP1; IPOA5; NPI-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Karyopherin Alpha 1 (KPNa1); Recombinant Karyopherin Alpha 1 (KPNa1); KPNa1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LC RGKSPPPEFA KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP KESIKKEACW TISNITAGNR AQIQTVIDAN IFPALISILQ TAEFRTRKEA AWAITNATSG GSAEQIKYLV ELGCIKPLCD LLTVMDSKIV QVALNGLENI LRLGEQEAKR NGTGINPYCA LIEEAYGLDK IEFLQSHENQ EIYQKAFDLI EHYFGT
• Sequence Length: 538

• Applicable Applications for KPNa1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu239~Thr506 (Accession # P52294) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 222144293
• NCBI GeneID: 3836
• NCBI Accession #: NP_002255.3
• NCBI GenBank Nucleotide #: NM_002264.3
• UniProt Accession #: P52294; Q6IBQ9; Q9BQ56; D3DN93
• Molecular Weight: 33.3kDa
• NCBI Official Full Name: importin subunit alpha-5
• NCBI Official Synonym Full Names: karyopherin alpha 1 (importin alpha 5)
• NCBI Official Symbol: KPNA1
• NCBI Official Synonym Symbols: RCH2; SRP1; IPOA5; NPI-1
• NCBI Protein Information: importin subunit alpha-5; SRP1-beta; importin alpha 5; importin-alpha-S1; RAG cohort protein 2; importin subunit alpha-1; nucleoprotein interactor 1; karyopherin subunit alpha-1; recombination activating gene cohort 2
• UniProt Protein Name: Importin subunit alpha-5
• UniProt Gene Name: KPNA1
• UniProt Synonym Gene Names: RCH2; NPI-1
• UniProt Entry Name: IMA5_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

KPNA1: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Belongs to the importin alpha family.

Protein type: Nuclear import; Karyopherin; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; dendrite; cytoplasm; nuclear pore; cytosol; nucleus

Molecular Function: protein binding; nuclear localization sequence binding; protein transporter activity

Biological Process: viral reproduction; positive regulation of protein import into nucleus; apoptosis; regulation of DNA recombination; cytokine and chemokine mediated signaling pathway; viral infectious cycle; DNA fragmentation during apoptosis; NLS-bearing substrate import into nucleus; cell structure disassembly during apoptosis