KLK-4 / Kallikrein-4 Recombinant Protein | KLK-4 recombinant protein

Recombinant Human KLK-4 / Kallikrein-4 Protein (His tag)

Cat. Num. AAA2545647

• Gene Names: KLK4; ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: KLK-4 / Kallikrein-4; Recombinant Human KLK-4 / Kallikrein-4 Protein (His tag); AI2A1; ARM1; EMSP; EMSP1; KLK-L1; MGC116827; MGC116828; PRSS17; PSTS; KLK-4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 254

• Application Notes: The secreted recombinant human KLK4 consists of 239 amino acids and predictes a molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK4 is approximately 30-35 kDa due to glycosylation.
• Predicted N Terminal: Ser 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for KLK-4 recombinant protein

Background: Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17 and EMSP1, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains one peptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20). The late protease is kallikrein 4 (KLK4). The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

Description: A DNA sequence encoding the human KLK4 (NP_004908.3) (Met 1-Ser 254) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 89142741
• NCBI GeneID: 9622
• NCBI Accession #: NP_004908.3
• NCBI GenBank Nucleotide #: NM_004917.3
• Molecular Weight: 11,858 Da
• NCBI Official Full Name: kallikrein-4 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 4
• NCBI Official Symbol: KLK4
• NCBI Official Synonym Symbols: ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• NCBI Protein Information: kallikrein-4
• UniProt Protein Name: Kallikrein-4
• UniProt Gene Name: KLK4
• UniProt Synonym Gene Names: EMSP1; PRSS17; PSTS; KLK-L1

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Several transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Molecular Function: protein binding

Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1