Rat Kallikrein 4 (KLK4) ELISA Kit | KLK4 elisa kit

Rat Kallikrein 4 (KLK4) ELISA Kit

Cat. Num. AAA2024527

• Gene Names: KLK4; ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• Reactivity: Rat
• Synonyms: Kallikrein 4 (KLK4); Rat Kallikrein 4 (KLK4) ELISA Kit; ARM1; EMSP1; KLK-L1; PRSS17; PSTS; Enamel Matrix; Prostate; Kallikrein-Related Peptidase 4; Enamel matrix serine proteinase 1; Serine protease 17; Kallikrein-like protein 1; KLK4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kallikrein 4 (KLK4).; No significant cross-reactivity or interference between Kallikrein 4 (KLK4) and analogues was observed.
• Sequence Length: 110

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.049ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 4 (KLK4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 4 (KLK4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 4 (KLK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 4 (KLK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 4 (KLK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 4 (KLK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KLK4 elisa kit

Enzyme & Kinase; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 89142741
• NCBI GeneID: 9622
• NCBI Accession #: NP_004908.3
• NCBI GenBank Nucleotide #: NM_004917.3
• UniProt Accession #: Q9Y5K2; Q4VB16; Q96RU5; Q9GZL6; Q9UBJ6
• Molecular Weight: 11,858 Da
• NCBI Official Full Name: kallikrein-4 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 4
• NCBI Official Symbol: KLK4
• NCBI Official Synonym Symbols: ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• NCBI Protein Information: kallikrein-4
• UniProt Protein Name: Kallikrein-4
• Protein Family: Kallikrein
• UniProt Gene Name: KLK4
• UniProt Synonym Gene Names: EMSP1; PRSS17; PSTS; KLK-L1
• UniProt Entry Name: KLK4_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Several transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Secreted; Protease

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; metal ion binding

Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis

Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1