Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1) Recombinant Protein | KIR2DL1 recombinant protein

Recombinant Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1)

Cat. Num. AAA2011879

• Gene Names: KIR2DL1; NKAT; NKAT1; p58.1; CD158A; KIR221; KIR-K64
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1); Recombinant Killer Cell Immunoglobulin Like Receptor 2DL1 (KIR2DL1); KIR2DL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- HEGVHRKPS LLAHPGPLVK SEETVILQCW SDVMFEHFLL HREGMFNDTL RLIGEHHDGV SKANFSISRM TQDLAGTYRC YGSVTHSPYQ VSAPSDPLDI VIIGLYEKPS LSAQPGPTVL AGENVTLSCS SRSSYDMYHL SREGEAHERR LPAGPKVNGT FQADFPLGPA THGGTYRCFG SFHDSPYEWS KSSDPLLVSV TGNPSNSWPS PTEPSSKTGN PRHLHILIGT SVVIILFILL FFLLHRWCSN KKNAAVMDQE SAGNRTANSE DSDEQDPQEV TYTQLNHCVF TQRKITRPSQ RPKTPPTDII VYTELPNAESRSKVVSCP TQRKITRPSQ RPKTPPTDII VYTELPNAES RSKVVSCP
• Sequence Length: 348

• Applicable Applications for KIR2DL1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: His22~Pro348 (Accession # P43626) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110611923
• NCBI GeneID: 3802
• NCBI Accession #: NP_055033.2
• NCBI GenBank Nucleotide #: NM_014218.2
• UniProt Accession #: P43626; O43470; Q32WE6
• Molecular Weight: 68.2kDa
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 2DL1
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
• NCBI Official Symbol: KIR2DL1
• NCBI Official Synonym Symbols: NKAT; NKAT1; p58.1; CD158A; KIR221; KIR-K64
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL1; NKAT-1; killer Ig receptor; p58 NK receptor CL-42/47.11; MHC class I NK cell receptor; killer inhibitory receptor 2-2-1; CD158 antigen-like family member A; natural killer-associated transcript 1; p58 NK cell inhibitory receptor NKR-K6; p58.1 MHC class-I-specific NK receptor; p58 killer cell inhibitory receptor KIR-K64; p58 natural killer cell receptor clones CL-42/47.11
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL1
• Protein Family: Killer cell immunoglobulin-like receptor
• UniProt Gene Name: KIR2DL1
• UniProt Synonym Gene Names: CD158A; NKAT1; NKAT-1
• UniProt Entry Name: KI2L1_HUMAN

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

KIR2DL1: Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. Interacts with ARRB2. Interacts with PTPN6; the interaction is enhanced by ARRB2. Interacts with PTPN11; the interaction is enhanced by ARRB2. Belongs to the immunoglobulin superfamily.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: regulation of immune response; immune response; natural killer cell inhibitory signaling pathway