SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Kell recombinant protein
Recombinant mouse Kell protein
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Kell; Recombinant mouse Kell protein; kell blood group antigen; kell blood group glycoprotein; Kell blood group; Kell blood group glycoprotein homolog; KEL; CD238 antigen; CD238; ECE3; Kell blood group-metalloendopeptidase; Kell blood group-metallo-endopeptidase; Kell recombinant protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
49-713aa
Sequence
Applicable Applications for Kell recombinant protein
SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Ala49
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for Kell recombinant protein
Kell, also known as Kell blood group glycoprotein homolog, is a member of the zinc endopeptidase of the neprilysin (NEP) family. It is an enzyme whose principal known function is the production of a potent bioactive peptide, ET-3. It also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins. It is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens. The Kell antigen system (also known as Kell-Cellano system) is one of the most clinically important blood group systems. Recombinant mouse Kell, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Kell recombinant protein
References
Lee, S. et al. (1999) Blood 94:1440-1450. Lee, S. et al. (2000) Transfus. Med. Rev. 14:93-103. Turner, A.J. et al. (2001) BioEssays. 23:261-269.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
76.3kDa (674aa)
UniProt Protein Name
Kell blood group glycoprotein homolog
Protein Family
UniProt Gene Name
Kel
UniProt Entry Name
KELL_MOUSE
Uniprot Description
Function: Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3
By similarity. Ref.1
Cofactor: Binds 1 zinc ion per subunit
By similarity.
Subunit structure: Heterodimer with XK; disulfide-linked. Ref.1
Subcellular location: Cell membrane; Single-pass type II membrane protein
By similarity. Note: Spans the erythrocyte membrane, and is attached to the underlying cytoskeleton
By similarity.
Tissue specificity: Highly expressed in spleen. Weaker expression in testis and heart. Ref.1
Post-translational modification: N-glycosylated. Ref.1
Sequence similarities: Belongs to the peptidase M13 family.
Similar Products
Product Notes
The Kell kel (Catalog #AAA201268) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 49-713aa. AAA Biotech's Kell can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Kell kel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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