Rabbit anti-Human, Mouse MAK Polyclonal Antibody | anti-mak antibody

MAK, CT (Male Germ Cell-associated Kinase, Serine/threonine-protein Kinase MAK, dJ417M14.2)

Cat. Num. AAA6003418

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAK; Polyclonal Antibody; CT (Male Germ Cell-associated Kinase; Serine/threonine-protein Kinase MAK; dJ417M14.2); Anti -MAK; anti-mak antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-mak antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAK (arrow) using MAK Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(MAK Antibody (T593) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the MAK antibody detected the MAK protein (arrow).)

Related Product Information for anti-mak antibody

MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.

Product Categories/Family for anti-mak antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 291616502
• NCBI GeneID: 8894794
• NCBI Accession #: YP_003519244.1
• NCBI GenBank Nucleotide #: NC_013956.2
• NCBI Official Full Name: Mak
• NCBI Official Symbol: mak
• NCBI Protein Information: Probable manno(fructo)kinase; Mak
• Protein Family: Makatoxin