Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) Recombinant Protein | VEGFR2 recombinant protein

Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)

Cat. Num. AAA2012116

• Gene Names: KDR; FLK1; CD309; VEGFR; VEGFR2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); VEGFR2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-NTTLQ ITCRGQRDLD WLWPNNQSGS EQRVEVTECS DGLFCKTLTI PKVIGNDTGA YKCFYRETDL ASVIYVYVQD YRSPFIASVS DQHGVVYITE NKNKTVVIPC LGSISNLNVS LCARYPEKRF VPDGNRISWD SKKGFTIPSY MISYAGMVFC EAKINDESYQ SIMYIVVVVG YRIYDVVLSP SHGIELSVGE KLVLNCTART ELNVGIDFNW EYPSSKHQHK KLVNRDLKTQ SGSEMKKFLS TLTIDGVTRS DQGLYTCAAS SGLMTKKNST
• Sequence Length: 1356

• Applicable Applications for VEGFR2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn46~Thr320 (Accession # P35968) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 11321597
• NCBI GeneID: 3791
• NCBI Accession #: NP_002244.1
• NCBI GenBank Nucleotide #: NM_002253.2
• UniProt Accession #: P35968; O60723; Q14178; A2RRS0; B5A925; C5IFA0
• Molecular Weight: 32.4kDa
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor (a type III receptor tyrosine kinase)
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK1; CD309; VEGFR; VEGFR2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: KDR
• UniProt Synonym Gene Names: FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
• UniProt Entry Name: VGFR2_HUMAN

NCBI Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; EC 2.7.10.1; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 4q11-q12

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; early endosome; cytoplasmic membrane-bound vesicle; plasma membrane; extracellular region; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; Hsp90 protein binding; ATP binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; ovarian follicle development; positive regulation of focal adhesion formation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of cell migration

Disease: Hemangioma, Capillary Infantile