Rat kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit | KDR elisa kit

Rat Vascular endothelial cell growth factor receptor 2, VEGFR-2/Flk-1 ELISA Kit

Cat. Num. AAA705176

• Gene Names: Kdr; Vegfr-2
• Reactivity: Rat
• Synonyms: kinase insert domain receptor (a type III receptor tyrosine kinase); Rat Vascular endothelial cell growth factor receptor 2; VEGFR-2/Flk-1 ELISA Kit; VEGFR-2/Flk-1 ELISA kit; CD309; FLK1; VEGFR; VEGFR2; fetal liver kinase-1; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; vascular endothelial growt; KDR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat KDR. No significant cross-reactivity or interference between Rat KDR and analogues was observed.
• Sequence Length: 1343

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Angiogenesis
• Protein Biological Process 3: Angiogenesis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KDR elisa kit

Introduction: Vascular endothelial cell growth factor (VEGF), also known as vascular permeability factor, is an endothelial cell mitogen, which stimulates angiogenesis by binding to specific receptors (VEGFRs) on the surface of vascular endothelial cells. The VEGF ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. All the three receptors for VEGF, VEGFR1~3, belong to CSF-1/PDGF receptor subfamily of receptor tyrosine kinase superfamily (RTKs), and contains seven Ig-like C2-type domains in the ECD, as well as a intracellular protein kinase domain. VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. The adaptor protein SHB has been shown to interact with VEGFR2 in receptor tyrosine kinase signaling. In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFR-2/Flk-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VEGFR-2/Flk-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VEGFR-2/Flk-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VEGFR-2/Flk-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981128
• NCBI GeneID: 25589
• NCBI Accession #: NP_037194.1
• NCBI GenBank Nucleotide #: NM_013062.1
• UniProt Accession #: O08775
• Molecular Weight: 150,394 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Vegfr-2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; FLK-1; fetal liver kinase 1; kinase insert domain protein receptor; protein-tyrosine kinase receptor flk-1; FLK1 kinase insert domain receptor (VEGF receptor 2); FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2)
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk1; VEGFR-2; FLK-1
• UniProt Entry Name: VGFR2_RAT

NCBI Description

receptor for vascular endothelial growth factor (VEGF) [RGD, Feb 2006]

Uniprot Description

Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. Binding of VEGFA, VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. May be regulated by hydrogen sulfide (H2S) levels via a sensitive intracellular disulfide bond