Mouse anti-Human KDM3A Monoclonal Antibody | anti-KDM3A antibody

KDM3A Antibody

Cat. Num. AAA9602574

• Gene Names: KDM3A; TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: KDM3A; Monoclonal Antibody; KDM3A Antibody; DKFZp686A24246; DKFZp686P07111; JHDM2A; JHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; Jumonji C domain containing histone demethylase 2A; Jumonji domain containing 1; Jumonji domain containing 1A; Jumonji domain containing protein 1A; Jumonji domain-containing protein 1A; Kdm3a; KDM3A_HUMAN; KIAA0742; Lysine (K) specific demethylase 3A; Lysine-specific demethylase 3A; Testis specific protein A; TSGA; anti-KDM3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: KDM3A antibody detects endogenous levels of total KDM3A
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1321

• Applicable Applications for anti-KDM3A antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000
• Immunogen: Purified recombinant fragment of human KDM3A expressed in E. Coli
• Subcellular Location: Cytoplasmic and Nuclear
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using KDM3A mouse mAb against Hela (1) and HepG2 (2) cell lysate.)

Related Product Information for anti-KDM3A antibody

Description: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono-and dimethylation-specific demethylase, binding iron and 劥-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.
Function: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono-and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Similarity: The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors. Belongs to the JHDM2 histone demethylase family.

NCBI and Uniprot Product Information

• NCBI GI #: 226442779
• NCBI GeneID: 55818
• NCBI Accession #: NP_001140160.1
• NCBI GenBank Nucleotide #: NM_001146688.1
• UniProt Accession #: Q9Y4C1; Q53S72; Q68D47; Q68UT9; Q6N050; Q8IY08; D6W5M3
• Molecular Weight: Observed: 147 kDa; Predicted: 148 kDa
• NCBI Official Full Name: lysine-specific demethylase 3A
• NCBI Official Synonym Full Names: lysine demethylase 3A
• NCBI Official Symbol: KDM3A
• NCBI Official Synonym Symbols: TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• NCBI Protein Information: lysine-specific demethylase 3A
• UniProt Protein Name: Lysine-specific demethylase 3A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM3A
• UniProt Synonym Gene Names: JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA

NCBI Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.