Bovine Aquaporin-2 ELISA Kit | AQP2 elisa kit

Bovine Aquaporin-2 ELISA Kit

Cat. Num. AAA288245

• Reactivity: Bovine
• Synonyms: Aquaporin-2; Bovine Aquaporin-2 ELISA Kit; AQP2 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural Bovine AQP-2. No significant cross-reactivity or interference was observed.
• Sequence Length: 271

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Competitive
• Detection Range: 0.31-20.0 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine AQP-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP2 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-2, During the reaction, AQP-2 in the sample or standard competes with a fixed amount of biotin-labeled AQP-2 for sites on a pre-coated Monoclonal antibody specific to AQP-2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AQP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 155372119
• NCBI GeneID: 539870
• NCBI Accession #: NP_001094669.1
• NCBI GenBank Nucleotide #: NM_001101199.1
• UniProt Accession #: P79099; A7MBE5
• Molecular Weight: 28,890 Da
• NCBI Official Full Name: aquaporin-2
• NCBI Official Symbol: AQP2
• NCBI Protein Information: aquaporin-2
• UniProt Protein Name: Aquaporin-2
• Protein Family: Aquaporin
• UniProt Gene Name: AQP2
• UniProt Synonym Gene Names: AQP-2; AQP-CD
• UniProt Entry Name: AQP2_BOVIN

Uniprot Description

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.