Typical Testing Data/Standard Curve (for reference only)
Mouse Haptoglobin ELISA Kit | Hp elisa kit
Mouse Haptoglobin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Haptoglobin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Haptoglobin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Haptoglobin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Haptoglobin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
HPR: a haptoglobin-related protein that binds hemoglobin as efficiently as haptoglobin. Unlike haptoglobin, plasma concentration of this protein is unaffected in patients with sickle cell anemia and extensive intravascular hemolysis, suggesting a difference in binding between haptoglobin-hemoglobin and haptoglobin-related protein-hemoglobin complexes to CD163, the hemoglobin scavenger receptor. This protein may also be a clinically important predictor of recurrence of breast cancer. [provided by RefSeq, Oct 2011]
Protein type: Secreted, signal peptide; Secreted
Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus
Molecular Function: antioxidant activity; hemoglobin binding; protein homodimerization activity; serine-type endopeptidase activity
Biological Process: acute-phase response; defense response to bacterium; immune system process; liver development; negative regulation of oxidoreductase activity; Notch signaling pathway; proteolysis; response to hydrogen peroxide
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Product Notes
The Mouse Hp hp (Catalog #AAA2882659) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882659 ELISA Kit recognizes Mouse Hp. It is sometimes possible for the material contained within the vial of "Haptoglobin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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