Typical Testing Data/Standard Curve (for reference only)
Mouse Lysyl tRNA Synthetase ELISA Kit | KARSL elisa kit
Mouse KARSL (Lysyl tRNA Synthetase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KARSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KARS.You can calculate the concentration of KARSin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; EC 6.1.1.6; Translation; Ligase
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; extracellular region; microtubule cytoskeleton; mitochondrial matrix; mitochondrion; nucleus; plasma membrane
Molecular Function: amino acid binding; ATP binding; lysine-tRNA ligase activity; metal ion binding; protein binding; tRNA binding
Biological Process: diadenosine tetraphosphate biosynthetic process; gene expression; lysyl-tRNA aminoacylation; selenium metabolic process; tRNA aminoacylation for protein translation; tRNA processing; viral reproduction
Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89
Research Articles on KARSL
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Product Notes
The Mouse KARSL kars (Catalog #AAA2533484) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533484 ELISA Kit recognizes Mouse KARSL. It is sometimes possible for the material contained within the vial of "Lysyl tRNA Synthetase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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