Human Lysyl tRNA Synthetase (KARS) ELISA Kit | KARS elisa kit

Human Lysyl tRNA Synthetase (KARS) ELISA Kit

Cat. Num. AAA454113

• Gene Names: KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89
• Reactivity: Human
• Synonyms: Lysyl tRNA Synthetase (KARS); Human Lysyl tRNA Synthetase (KARS) ELISA Kit; KARS2; LysRS; Lysine tRNA Ligase; KARS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KARS. No significant cross-reactivity or interference between KARS and analogues was observed.
• Sequence Length: 625

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.082ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KARS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KARS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KARS elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of KARS in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KARS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194272210
• NCBI GeneID: 3735
• NCBI Accession #: NP_001123561.1
• NCBI GenBank Nucleotide #: NM_001130089.1
• UniProt Accession #: Q15046; O14946; Q96J25; Q9HB23; A8MSK1; D3DUK4
• Molecular Weight: 71,497 Da
• NCBI Official Full Name: lysine--tRNA ligase isoform 1
• NCBI Official Synonym Full Names: lysyl-tRNA synthetase
• NCBI Official Symbol: KARS
• NCBI Official Synonym Symbols: KRS; KARS1; KARS2; CMTRIB; DFNB89
• NCBI Protein Information: lysine--tRNA ligase
• UniProt Protein Name: Lysine--tRNA ligase
• UniProt Gene Name: KARS
• UniProt Synonym Gene Names: KIAA0070; LysRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA3(Lys), the primer for reverse transcription initiation.