Lysine--tRNA Ligase/KARS Recombinant Protein | KARS recombinant protein

Recombinant Human Lysine--tRNA Ligase/KARS Protein (C-6His)

Cat. Num. AAA2552929

• Gene Names: KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Lysine--tRNA Ligase/KARS; Recombinant Human Lysine--tRNA Ligase/KARS Protein (C-6His); Lysine--tRNA Ligase; Lysyl-tRNA Synthetase; LysRS; KARS; KIAA0070; KARS recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM Tris HCl, 150mM NaCl, 1mM DTT, 20% Glycerol, pH 8.0.
• Sequence Positions: Ala2-Val597
• Sequence Length: 625

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Related Product Information for KARS recombinant protein

Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).

NCBI and Uniprot Product Information

• NCBI GI #: 194272210
• NCBI GeneID: 3735
• NCBI Accession #: NP_001123561.1
• NCBI GenBank Nucleotide #: NM_001130089.1
• UniProt Accession #: Q15046; O14946; Q96J25; Q9HB23; A8MSK1; D3DUK4
• Molecular Weight: Molecular Mass: 69.1 kDa; Actual Protein Molecular Mass: 65 kDa
• NCBI Official Full Name: lysine--tRNA ligase isoform 1
• NCBI Official Synonym Full Names: lysyl-tRNA synthetase
• NCBI Official Symbol: KARS
• NCBI Official Synonym Symbols: KRS; KARS1; KARS2; CMTRIB; DFNB89
• NCBI Protein Information: lysine--tRNA ligase
• UniProt Protein Name: Lysine--tRNA ligase
• UniProt Gene Name: KARS
• UniProt Synonym Gene Names: KIAA0070; LysRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).