Mouse Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit | Itih3 elisa kit

Mouse Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit

Cat. Num. AAA762790

• Gene Names: ITIH3; H3P; SHAP; ITI-HC3
• Reactivity: Mouse
• Synonyms: Inter-alpha-trypsin inhibitor heavy chain H3; Mouse Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit; Itih3/Inter-alpha-trypsin inhibitor heavy chain H3; Itih3 elisa kit

• Reactivity: Mouse
• Sequence Length: 890

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Itih3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Itih3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Itih3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Itih3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Itih3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 133925809
• NCBI GeneID: 3699
• NCBI Accession #: NP_002208.3
• NCBI GenBank Nucleotide #: NM_002217.3
• Molecular Weight: 99,328 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain 3
• NCBI Official Symbol: ITIH3
• NCBI Official Synonym Symbols: H3P; SHAP; ITI-HC3
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H3
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H3
• Protein Family: Inter-alpha-trypsin inhibitor
• UniProt Gene Name: ITIH3
• UniProt Synonym Gene Names: ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP
• UniProt Entry Name: ITIH3_HUMAN

NCBI Description

This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]

Uniprot Description

ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process