Goat LNK Polyclonal Antibody | anti-LNK antibody

GOAT ANTI HUMAN LNK (N-TERMINAL)

Cat. Num. AAA221312

• Gene Names: SH2B3; LNK; IDDM20
• Applications: Western Blot
• Synonyms: LNK; Polyclonal Antibody; GOAT ANTI HUMAN LNK (N-TERMINAL); anti-LNK antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 575

• Applicable Applications for anti-LNK antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 70kDa in human MOLT4 cell lysates. (Predicted Mwt. 63.2kDa). A band of 68kDA was resolved in HUVECs, See Fitau et al. for details.; Western Blotting: Minimum Dilution: 0.3; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin; Preparation
• Buffer Solution: TRIS buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blotting analysis of MOLT4 lysate probed with Goat anti Human LNK (MBS221312))

Testing Data #2

(Western blotting analysis of HEK293 cells transiently transfected with human Lnk probed with Goat anti Human LnkLane A: Mock transfected cells. Lane B: transfected cells)

Related Product Information for anti-LNK antibody

Goat anti Human LNK antibodyrecognizes human SH2B adapter protein 3, also known as Lymphocyte-specific adapter protein Lnk or Lymphocyte adapter protein. LNK is a 575 amino acid SH2B adapteer protein containing a single PH domain and a single SH2 domain. Goat anti Human LNK antibody binds to an epitope within the N-terminal region of LNK, which regulates cytokine receptor-mediated and growth factor pathways, and plays an essential role in B cell lymphopoiesis. LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFalpha. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFalpha, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 611435019
• NCBI GeneID: 10019
• NCBI Accession #: NP_001278353.1
• NCBI GenBank Nucleotide #: NM_001291424.1
• UniProt Accession #: Q9UQQ2; O95184; B9EGG5
• Molecular Weight: 63,225 Da
• NCBI Official Full Name: SH2B adapter protein 3 isoform 2
• NCBI Official Synonym Full Names: SH2B adaptor protein 3
• NCBI Official Symbol: SH2B3
• NCBI Official Synonym Symbols: LNK; IDDM20
• NCBI Protein Information: SH2B adapter protein 3; lymphocyte-specific adapter protein Lnk; signal transduction protein Lnk
• UniProt Protein Name: SH2B adapter protein 3
• UniProt Gene Name: SH2B3
• UniProt Synonym Gene Names: LNK
• UniProt Entry Name: SH2B3_HUMAN

NCBI Description

This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

LNK: Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase. Genetic variations in SH2B3 are associated with susceptibility to celiac disease type 13 (CELIAC13)[MIM:612011]; also known as susceptibility to gluten-sensitive enteropathy type 13. Celiac disease is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. Genetic variations in SH2B3 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM); also known as diabetes mellitus type 1. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis. Belongs to the SH2B adapter family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: cytosol

Molecular Function: protein binding; signal transducer activity; protein complex binding; phosphate binding

Biological Process: embryonic hemopoiesis; blood coagulation; cell differentiation

Disease: Myelofibrosis; Erythrocytosis, Familial, 1; Thrombocythemia 1