Human Integrin alpha-5 ELISA Kit | ITGA5 elisa kit

Human Integrin alpha-5 ELISA Kit

Cat. Num. AAA761812

• Gene Names: ITGA5; FNRA; CD49e; VLA-5; VLA5A
• Reactivity: Human
• Synonyms: Integrin alpha-5; Human Integrin alpha-5 ELISA Kit; ITGA5/CD49e/VLA-5 alpha/CD49 antigen-like family member E/CD49e/CD49e antigen/Fibronectin receptor subunit alpha/fibronectin receptor; alpha subunit/FNRAVLA5A/integrin alpha-5/Integrin alpha-F/integrin; alpha 5 (fibronectin receptor; alpha polypeptide)/very late activation protein 5; alpha subunit/VLA-5; ITGA5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGA5. No significant cross-reactivity or interference between ITGA5 and analogues was observed.
• Sequence Length: 1098

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGA5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 938148811
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.3
• NCBI GenBank Nucleotide #: NM_002205.3
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 5
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA-5; VLA5A
• NCBI Protein Information: integrin alpha-5
• UniProt Protein Name: Integrin alpha-5
• Protein Family: Integrin
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA
• UniProt Entry Name: ITA5_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Uniprot Description

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: cell surface; cytoplasmic vesicle; endoplasmic reticulum; external side of plasma membrane; focal adhesion; Golgi apparatus; integrin complex; intercellular junction; plasma membrane; ruffle; synapse

Molecular Function: epidermal growth factor receptor binding; integrin binding; metal ion binding; platelet-derived growth factor receptor binding; protein binding; vascular endothelial growth factor receptor 2 binding; viral receptor activity

Biological Process: angiogenesis; axon guidance; blood coagulation; cell adhesion; cell-cell adhesion mediated by integrin; cell-substrate adhesion; cell-substrate junction assembly; entry of virus into host cell; extracellular matrix organization and biogenesis; heterophilic cell adhesion; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; memory; positive regulation of cell migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; wound healing, spreading of epidermal cells