Mouse Integrin alpha-IIb ELISA Kit | Itga2b elisa kit

Mouse Integrin alpha-IIb ELISA Kit

Cat. Num. AAA765489

• Gene Names: ITGA2B; GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
• Reactivity: Mouse
• Synonyms: Integrin alpha-IIb; Mouse Integrin alpha-IIb ELISA Kit; Itga2b/CD41 (Integrin alpha-Iib)/GP2B/GPIIb/GTA/HPA3/CD41 antigen/GPalpha IIb/ITGAB/platelet fibrinogen receptor; alpha subunit/Platelet membrane glycoprotein IIb/platelet-specific antigen BAK; Itga2b elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Itga2b. No significant cross-reactivity or interference between Itga2b and analogues was observed.
• Sequence Length: 1039

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Itga2b elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 88758615
• NCBI GeneID: 3674
• NCBI Accession #: NP_000410.2
• NCBI GenBank Nucleotide #: NM_000419.4
• Molecular Weight: 103,218 Da
• NCBI Official Full Name: integrin alpha-IIb preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 2b
• NCBI Official Symbol: ITGA2B
• NCBI Official Synonym Symbols: GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
• NCBI Protein Information: integrin alpha-IIb
• UniProt Protein Name: Integrin alpha-IIb
• Protein Family: Integrin
• UniProt Gene Name: ITGA2B
• UniProt Synonym Gene Names: GP2B; ITGAB; GPIIb
• UniProt Entry Name: ITA2B_HUMAN

NCBI Description

This gene encodes a member of the integrin alpha chain family of proteins. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate through disulfide linkages to form a subunit of the alpha-IIb/beta-3 integrin cell adhesion receptor. This receptor plays a crucial role in the blood coagulation system, by mediating platelet aggregation. Mutations in this gene are associated with platelet-type bleeding disorders, which are characterized by a failure of platelet aggregation, including Glanzmann thrombasthenia. [provided by RefSeq, Jan 2016]

Uniprot Description

ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell surface; external side of plasma membrane; focal adhesion; integral to plasma membrane; integrin complex; plasma membrane; platelet alpha granule membrane

Molecular Function: extracellular matrix binding; identical protein binding; metal ion binding; protein binding

Biological Process: axon guidance; blood coagulation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; integrin-mediated signaling pathway; platelet activation; platelet degranulation; positive regulation of leukocyte migration

Disease: Bleeding Disorder, Platelet-type, 16; Glanzmann Thrombasthenia