Western Blot (WB)
((0.3ug/ml) staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat CSNK2B Polyclonal Antibody | anti-CSNK2B antibody
Goat anti-CSNK2B (aa172-186) Antibody
Western Blot: Approx 26kDa band observed in lysates of cell lines NIH3T3, HeLa, K562, MCF7 and HepG2 (calculated MW of 24.9kDa according to NP_001311.3). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.3ug/ml) staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), HeLa (B), K562 (C), MCF7 (D) and HepG2 (E) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
CK2B: a regulatory subunit of the casein kinase 2 holoenzyme. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. Participates in Wnt signaling
Protein type: Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; PcG protein complex; plasma membrane; protein kinase CK2 complex
Molecular Function: chromatin binding; identical protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase regulator activity; protein serine/threonine kinase activity; receptor binding; transcription factor binding
Biological Process: adiponectin-mediated signaling pathway; cellular protein complex assembly; negative regulation of blood vessel endothelial cell migration; negative regulation of cell proliferation; positive regulation of activin receptor signaling pathway; protein amino acid phosphorylation; protein folding; regulation of DNA binding; regulation of protein kinase activity; signal transduction; Wnt receptor signaling pathway
Research Articles on CSNK2B
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Product Notes
The CSNK2B csnk2b (Catalog #AAA423548) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CSNK2B (aa172-186) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CSNK2B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 26kDa band observed in lysates of cell lines NIH3T3, HeLa, K562, MCF7 and HepG2 (calculated MW of 24.9kDa according to NP_001311.3). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the CSNK2B csnk2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PEYRPKRPAN QFVPR. It is sometimes possible for the material contained within the vial of "CSNK2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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