2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ISPD) Recombinant Protein | ISPD recombinant protein

Recombinant Human 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ISPD)

Cat. Num. AAA1162650

• Gene Names: ISPD; Nip; hISPD; MDDGA7; MDDGC7; hCG_1745121
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ISPD); Recombinant Human 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ISPD); ISPD recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-451, Full length protein
• Sequence: MEAGPPGSARPAEPGPCLSGQRGADHTASASLQSVAGTEPGRHPQAVAAVLPAGGCGERMGVPTPKQFCPILERPLISYTLQALERVCWIKDIVVAVTGENMEVMKSIIQKYQHKRISLVEAGVTRHRSIFNGLKALAEDQINSKLSKPEVVIIHDAVRPFVEEGVLLKVVTAAKEHGAAGAIRPLVSTVVSPSADGCLDYSLERARHRASEMPQAFLFDVIYEAYQQCSDYDLEFGTECLQLALKYCCTKAKLVEGSPDLWKVTYKRDLYAAESIIKERISQEICVVMDTEEDNKHVGHLLEEVLKSELNHVKVTSEALGHAGRHLQQIILDQCYNFVCVNVTTSDFQETQKLLSMLEESSLCILYPVVVVSVHFLDFKLVPPSQKMENLMQIREFAKEVKERNILLYGLLISYPQDDQKLQESLRQGAIIIASLIKERNSGLIGQLLIA
• Sequence Length: 451

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 157671913
• NCBI GeneID: 729920
• NCBI Accession #: NP_001094887.1
• NCBI GenBank Nucleotide #: NM_001101417.3
• UniProt Accession #: A4D126; A8MU35; H9KVB2
• Molecular Weight: 44,389 Da
• NCBI Official Full Name: D-ribitol-5-phosphate cytidylyltransferase isoform b
• NCBI Official Synonym Full Names: isoprenoid synthase domain containing
• NCBI Official Symbol: ISPD
• NCBI Official Synonym Symbols: Nip; hISPD; MDDGA7; MDDGC7; hCG_1745121
• NCBI Protein Information: D-ribitol-5-phosphate cytidylyltransferase; isoprenoid synthase domain-containing protein
• UniProt Protein Name: D-ribitol-5-phosphate cytidylyltransferase
• Protein Family: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
• UniProt Gene Name: ISPD
• UniProt Synonym Gene Names: hISPD

NCBI Description

This gene encodes a 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein. Mutations in this gene are the cause of Walker-Warburg syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

Cytidylyltransferase required for protein O-linked mannosylation (PubMed:26687144, PubMed:27130732, PubMed:27601598, PubMed:22522420, PubMed:22522421). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (PubMed:26687144, PubMed:27130732). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:26687144, PubMed:27130732). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (PubMed:26687144). Not Involved in dolichol production (PubMed:26687144).