H1F0 / Histone H1 Recombinant Protein | H1F0 recombinant protein

Recombinant Human H1F0 / Histone H1 Protein (His tag)

Cat. Num. AAA2546012

• Gene Names: H1F0; H10; H1FV
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: H1F0 / Histone H1; Recombinant Human H1F0 / Histone H1 Protein (His tag); HSPD1; CPN60; GROEL; HLD4; HSP60; HSP65; HuCHA60; SPG13; H1F0 recombinant protein

• Host: E Coli
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 600mM NaCl, 1mM DTT, pH 8.5
• Sequence Length: 194

• Application Notes: The recombinant human H1F0 consisting of 205 amino acids and has a calculated molecular mass of 22.4 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for H1F0 recombinant protein

Background: H1 histone family, member 0 (H1F0) is a member of the H1 histone family of nuclear proteins which are a component of chromatin in eukaryotic cells. It's involved in maintaining the structure of chromatin by packing the "beads on a string" sub-structure into a high order structure. The lysine-rich H1 histone family in mammals includes eleven members. In higher eukaryotes all H1 variants have the same general structure, consisting of a central conserved globular domain and less conserved N-terminal and C-terminal tails. These tails are moderately conserved among species, but differ among variants, suggesting a specific function for each H1 variant. Studies on the role of particular subtype s at specific developmental stages in lower eukaryotes, but also in vertebrates suggest that specific subtype s of H1 participate in particular systems of gene regulation. 

Description: A DNA sequence encoding the human H1F0 (P07305) (Met 1-Lys 194) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4885371
• NCBI GeneID: 3005
• NCBI Accession #: NP_005309.1
• NCBI GenBank Nucleotide #: NM_005318.3
• UniProt Accession #: P07305; Q6FG88; Q8N6R3; B2R6I0; B4DRD6
• Molecular Weight: 19,167 Da
• NCBI Official Full Name: histone H1.0
• NCBI Official Synonym Full Names: H1 histone family member 0
• NCBI Official Symbol: H1F0
• NCBI Official Synonym Symbols: H10; H1FV
• NCBI Protein Information: histone H1.0
• UniProt Protein Name: Histone H1.0
• Protein Family: Histone
• UniProt Gene Name: H1F0
• UniProt Synonym Gene Names: H1FV

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]

Uniprot Description

H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: actin cytoskeleton; Golgi apparatus; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: chromatin DNA binding; protein binding

Biological Process: DNA fragmentation during apoptosis