Grancalcin Recombinant Protein | GCA recombinant protein

Recombinant Grancalcin (GCA)

Cat. Num. AAA2033265

• Gene Names: GCA; GCL
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Grancalcin; Recombinant Grancalcin (GCA); GCL; EF Hand Calcium Binding Protein; GCA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTV
• Sequence Length: 217

• Applicable Applications for GCA recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 18.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Val139
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6912388
• NCBI GeneID: 25801
• NCBI Accession #: NP_036330.1
• NCBI GenBank Nucleotide #: NM_012198.3
• Molecular Weight: 24,010 Da
• NCBI Official Full Name: grancalcin
• NCBI Official Synonym Full Names: grancalcin
• NCBI Official Symbol: GCA
• NCBI Official Synonym Symbols: GCL
• NCBI Protein Information: grancalcin
• UniProt Protein Name: Grancalcin
• Protein Family: Grancalcin
• UniProt Gene Name: GCA
• UniProt Synonym Gene Names: GCL
• UniProt Entry Name: GRAN_HUMAN

NCBI Description

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq, Jul 2008]

Uniprot Description

GCA: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: proteolysis