Human Interleukin-1 ELISA Kit | IRAK1 elisa kit

Human Interleukin-1 receptor-associated kinase 1 (IRAK1) ELISA Kit

Cat. Num. AAA285078

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human
• Synonyms: Interleukin-1; Human Interleukin-1 receptor-associated kinase 1 (IRAK1) ELISA Kit; IRAK; pelle; Pelle homolog; IRAK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human IRAK1. No significant cross-reactivity or interference between Human IRAK1 and analogues was observed.
• Sequence Length: 152

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for IRAK1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IRAK1 in samples. An antibody specific for IRAK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIRAK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IRAK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IRAK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 68800343
• NCBI GeneID: 3654
• NCBI Accession #: NP_001020413.1
• NCBI GenBank Nucleotide #: NM_001025242.1
• UniProt Accession #: P51617; Q7Z5V4; Q96C06; Q96RL2; D3DWW3; D3DWW4
• Molecular Weight: 68,022 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 2
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: EC 2.7.11.1; IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; cytosol; endosome membrane; interleukin-1 receptor complex; lipid droplet; nucleus; plasma membrane

Molecular Function: ATP binding; heat shock protein binding; kinase activity; NF-kappaB-inducing kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB-inducing kinase; aging; cellular response to heat; cellular response to hypoxia; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of NF-kappaB transcription factor activity; nucleotide-binding oligomerization domain containing signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon type I production; positive regulation of MAP kinase activity; positive regulation of NF-kappaB transcription factor activity; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; protein autophosphorylation; protein oligomerization; regulation of cytokine-mediated signaling pathway; response to lipopolysaccharide; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway