HVEM-Fc active protein

Human HVEM-Fc

Cat. Num. AAA691900

• Gene Names: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• Reactivity: Human
• Purity: > 98%, by SDS-PAGE and HPLC analysis
• Synonyms: HVEM-Fc; Human HVEM-Fc; Recombinant Human sHVEM/Fc Chimera; HVEM-Fc active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 98%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYI AHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI V QDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGT LEE CQHQTKRSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MI SRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNST YR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPR EPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENN Y KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNH YTQKSL SLSPGK
• Sequence Length: 283

• Endotoxin Level: < 0.1 ng per g sHVEM/Fc
• Biological Activity: Determined by its ability to neutralize 0.25 ng/ml of hTNFbeta induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/ml of HVEM-Fc.

Related Product Information for HVEM-Fc active protein

HVEM belongs to the TNF Receptor superfamily of transmembrane proteins and plays a role in the activation of T-cells and other lymphocytes. It is expressed in various cells and tissues including spleen, thymus, lung, macrophages, and T-cells. HVEM activation induces a signaling cascade which results in induction of transcription factors NF-kappaB and AP-1. LIGHT (TNFSF14) and TNF-beta (TNFSF1) function as the ligands for HVEM, which can also bind specifically to herpes simplex virus glycoprotein D. Soluble HVEM can act as a "receptor decoy" resulting in inhibition of the activity of the HVEM ligands, LIGHT and TNF-beta. Recombinant human HVEM-Fc Chimera is a 376 amino acid fusion protein containing an N-terminal domain corresponding to the extracellular region of HVEM and a C-terminal domain corresponding to residues 102 to 330 of human IgG1.

Product Categories/Family for HVEM-Fc active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 23200041
• NCBI GeneID: 8764
• NCBI Accession #: NP_003811.2
• NCBI GenBank Nucleotide #: NM_003820.2
• UniProt Accession #: Q92956; Q6IB95; Q8WXR1; Q96J31; Q9UM65; B9DI89
• Molecular Weight: 30,392 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 14
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 14
• NCBI Official Symbol: TNFRSF14
• NCBI Official Synonym Symbols: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14; CD40-like protein; herpesvirus entry mediator A; herpes virus entry mediator A; tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• UniProt Gene Name: TNFRSF14
• UniProt Synonym Gene Names: HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
• UniProt Entry Name: TNR14_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. Ref.1

Subunit structure: Interacts with TRAF2, TRAF3 and TRAF5. Interacts with herpes simplex virus 1 (HHV-1) and herpes simplex virus 1 (HHV-2) envelope glycoprotein D; functions as an entry receptor for these viruses. Ref.12 Ref.13 Ref.14

Subcellular location: Membrane; Single-pass type I membrane protein

Probable.

Tissue specificity: Widely expressed, with the highest expression in lung, spleen and thymus.

Post-translational modification: N-glycosylated. Ref.16

Sequence similarities: Contains 3 TNFR-Cys repeats.