MYLK3 active protein
Recombinant Human MYLK3 (N-GST tag)
Gene Names
MYLK3; MLCK; MLCK2; caMLCK
Purity
70%-90%
Synonyms
MYLK3; Recombinant Human MYLK3 (N-GST tag); caMLCK; MLCK; MLCK2; MGC126319; MGC126320; MYLK3 active protein
Host
Insect Cells
Purity/Purification
70%-90%
Form/Format
Liquid; 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Sequence Length
2624
Bioactivity
The specific activity of MYLK3 was determined to be 9 nmol/min/mg as per activity assay protocol.
Preparation and Storage
Store product at-70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Related Product Information for MYLK3 active protein
MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure. Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly. Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium-and calmodulin-dependent.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
135.0 kDa
NCBI Official Full Name
Homo sapiens myosin light chain kinase 3, mRNA
NCBI Official Synonym Full Names
myosin light chain kinase 3
NCBI Official Symbol
MYLK3
NCBI Official Synonym Symbols
MLCK; MLCK2; caMLCK
NCBI Protein Information
myosin light chain kinase 3
Protein Family
NCBI Description
Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]
Research Articles on MYLK3
Similar Products
Product Notes
The MYLK3 (Catalog #AAA4160241) is an Active Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MYLK3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
