Porcine Inducible nitric oxide synthase ELISA Kit | iNOS elisa kit

Porcine Inducible nitric oxide synthase ELISA Kit

Cat. Num. AAA747545

• Reactivity: Porcine
• Synonyms: Inducible nitric oxide synthase; Porcine Inducible nitric oxide synthase ELISA Kit; iNOS elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of iNOS. No significant cross-reactivity or interference between iNOS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iNOS and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for iNOS elisa kit

Intended Uses: This iNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine iNOS. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: iNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iNOS antibody and an iNOS-HRP conjugate. The assay sample and buffer are incubated together with iNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iNOS concentration since iNOS from samples and iNOS-HRP conjugate compete for the anti-iNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for iNOS elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 2935553
• Molecular Weight: 130,628 Da
• NCBI Official Full Name: inducible nitric oxide synthase
• UniProt Protein Name: Nitric oxide synthase, inducible
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inducible NOS; iNOS
• UniProt Entry Name: NOS2_RAT

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; vesicle membrane; nucleus; cytosol

Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; beta-catenin binding; cAMP-dependent protein kinase regulator activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; amino acid binding; FMN binding; NADPH-hemoprotein reductase activity; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding

Biological Process: retinoic acid receptor signaling pathway; wound healing; cGMP-mediated signaling; positive regulation of apoptosis; response to hormone stimulus; response to lipopolysaccharide; positive regulation of killing of cells of another organism; signal transduction; positive regulation of vasodilation; defense response to Gram-negative bacterium; regulation of heart contraction; response to estradiol stimulus; regulation of blood pressure; negative regulation of blood pressure; nitric oxide biosynthetic process; inflammatory response; glycoside metabolic process; aging; superoxide metabolic process; positive regulation of guanylate cyclase activity; ovulation from ovarian follicle; regulation of cell proliferation; response to mechanical stimulus; endothelial cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; G-protein signaling, coupled to cGMP nucleotide second messenger; blood vessel remodeling; response to activity; regulation of protein kinase activity; negative regulation of protein catabolic process; regulation of insulin secretion; nitric oxide mediated signal transduction