Typical Testing Data/Standard Curve (for reference only)
Rat inducible nitric oxide synthase, iNOS/NOS2 ELISA Kit | iNOS/NOS2 elisa kit
Rat inducible nitric oxide synthase, iNOS/NOS2 ELISA Kit
Reactivity
Rat
Synonyms
inducible nitric oxide synthase; iNOS/NOS2; Rat inducible nitric oxide synthase; iNOS/NOS2 ELISA Kit; iNOS/NOS2 elisa kit
Reactivity
Rat
Sequence Length
1147
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.2ng/ml-70ng/ml
Sensitivity
0.081ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for iNOS/NOS2 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Hypoxanthine-guanine Phosphoribosyltransferase (also known as HPRT1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HPRT1 antibody. HPRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HPRT1 Antibody is added and binds to HPRT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HPRT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HPRT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HPRT1 antibody. HPRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HPRT1 Antibody is added and binds to HPRT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HPRT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HPRT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
130,628 Da
NCBI Official Full Name
inducible nitric oxide synthase
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Gene Name
Nos2
UniProt Synonym Gene Names
Inducible NOS; iNOS
Uniprot Description
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
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Product Notes
The Rat iNOS/NOS2 nos2 (Catalog #AAA161069) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161069 ELISA Kit recognizes Rat iNOS/NOS2. It is sometimes possible for the material contained within the vial of "inducible nitric oxide synthase, iNOS/NOS2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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