K252a, Inhibitor
K252a
Purity
Highly Purified~98%
Synonyms
K252a; inhibitor
Purity/Purification
Highly Purified
~98%
~98%
Form/Format
Supplied as a lyophilized powder. Not sterile.
Biological Activity
PKA: Ki =18nM
PKG: Ki =20nM
MLCK: Ki =17nM
PKC: Ki =25nM
CaMK: Ki =1.8nM
Phosphorylase Kinase: IC50 =1.7nM
Trk: IC50 =3nM
PKG: Ki =20nM
MLCK: Ki =17nM
PKC: Ki =25nM
CaMK: Ki =1.8nM
Phosphorylase Kinase: IC50 =1.7nM
Trk: IC50 =3nM
Molecular Formula
C27H21N3O5
Solubility
DMSO (~1mg/ml); DMF (~1mg/ml)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with DMSO or DMF. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for K252a, inhibitor
K252a is an alkaloid isolated from Nocardiopsis sp. soil fungi. This compound is a highly potent cell permeable CaM Kinase II inhibitor (IC50=1.8nM). At higher concentrations it also inhibits broad spectrum of protein kinases 1-8. It is an excellent research tool of NGF receptors-trk-signal transduction due to its potent inhibition of trk-NGF receptor-tyrosine kinase activity.
K252a inhibits PKA, PKG, MLCK, PKC, CaMK, phosphorylase kinase, MAP kinase, the trk family of receptor tyrosine kinases and numerous other kinases by acting as a competitive inhibitor with respect to ATP. K252a binds to neuroblastoma membrane with high affinity (2.7nM). This compound is also observed to promote survival of primary neuronal cultures (75nM) and to induce neurite outgrowth in SH-SY5Y neuroblastoma cells, an event which is accompanied by enhanced phosphorylation of FAK.
K252a inhibits PKA, PKG, MLCK, PKC, CaMK, phosphorylase kinase, MAP kinase, the trk family of receptor tyrosine kinases and numerous other kinases by acting as a competitive inhibitor with respect to ATP. K252a binds to neuroblastoma membrane with high affinity (2.7nM). This compound is also observed to promote survival of primary neuronal cultures (75nM) and to induce neurite outgrowth in SH-SY5Y neuroblastoma cells, an event which is accompanied by enhanced phosphorylation of FAK.
Product Categories/Family for K252a, inhibitor
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Product Notes
The K252a (Catalog #AAA655447) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "K252a, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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