Dehydroeburicoic acid, Inhibitor
Dehydroeburicoic acid
Purity
98.00%
Synonyms
Dehydroeburicoic acid; inhibitor
Purity/Purification
98.00%
Form/Format
Solid. Powder
Solubility
<1mg/ml refers to the product slightly soluble or insoluble
Formula
C3lH48O3
SMILES
CC(C)C(=C)CC[C@H]([C@H]1CC[C@@]2([C@@]1(CC=C3C2=CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O)C)C)C)C(=O)O
CAS Number
6879-05-6
Target & IC50
NO Synthase
TNF-alpha
NOS
TNF-alpha
NOS
In Vitro
3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase release assays indicated that Dehydroeburicoic acid inhibited the proliferation of the human glioblastoma cell U87MG. In addition, Annexin V and propidium iodide staining showed that Dehydroeburicoic acid treatment, led to a rapid increase of glioblastomas in the necrotic/late apoptotic fraction, whereas cell cycle analysis revealed that Dehydroeburicoic acid failed to significantly enhance the population of U87MG cells in the hypodiploid (sub-G1) fraction. Using electron microscopy, we found that Dehydroeburicoic acid induced significant cell enlargements, massive cytoplasmic vacuolization, and loss of mitochondrial membrane integrity. Dehydroeburicoic acid treatment, triggered an intracellular Ca(2+) increase, and Dehydroeburicoic acid-induced cell death was significantly attenuated by BAPTA-AM but not ethylenediaminetetraacetic acid or ethylene glycol tetraacetic acid. Dehydroeburicoic acid instigated a reduction of both mitochondrial transmembrane potential and intracellular ATP level. Moreover, Dehydroeburicoic acid induced proteolysis of alpha-spectrin by calpain, and Dehydroeburicoic acid cytotoxicity in U87MG cells was caspase-independent but was effectively blocked by calpain inhibitor. Interestingly, Dehydroeburicoic acid also caused autophagic response that was prevented by calpain inhibitor.
Preparation and Storage
Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent
-80 degree C for 2 years in solvent
Related Product Information for Dehydroeburicoic acid, inhibitor
Dehydroeburicoic acid induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.
Product Categories/Family for Dehydroeburicoic acid, inhibitor
References
Dehydroeburicoic acid induces calcium- and calpain-dependent necrosis in human U87MG glioblastomas.Chem Res Toxicol. 2009 Nov;22(11):1817-26.
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Product Notes
The Dehydroeburicoic acid (Catalog #AAA5759836) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Dehydroeburicoic acid, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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