Caspase 3, Cleaved, Inhibitor | CASP3, inhibitor

Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1) Blocking Peptide

Cat. Num. AAA655367

• Gene Names: CASP3; CPP32; SCA-1; CPP32B
• Applications: ELISA
• Purity: Purified; Purified
• Synonyms: Caspase 3; Cleaved; Cleaved (Asp175) (CPP-32; Apoptain; Yama; SCA-1) Blocking Peptide; CASP3; inhibitor

• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 20mM potassium phosphate, pH 7.0, 50mM sodium chloride, 0.1mM EDTA, 1mg/ml BSA, 5% glycerol.

• Applicable Applications for CASP3, inhibitor: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight.; Dilution: Antibody Blocking (Western Blot): 10ul Peptide per 10ul antibody (C2087-16A) in 10ml Antibody Dilution Buffer. Incubate for 1 hour at RT prior to incubating with Immunoblot.; Antibody Blocking (Immunohistochemistry): 2ul Peptide per 2ul antibody (C2087-16A) in 96ul Antibody Dilution Buffer. Incubate for 2 hour at 4 degree C prior to incubating with Immunoblot.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: Cell extracts from Jurkat cells treated with 0.25mg/ml cytochrome c, using Caspase 3, Cleaved (Asp175) Pab Rb xHu|C2087-16A without preincubation (left) and preincubated with Caspase 3, Cleaved (Asp175)|C2087-16A1 as Blocking Peptide (right).)

Immunohistochemistry (IHC)

(Immunohistochemistery: Staining of paraffin-embedded human tonsil, using Caspase 3, Cleaved (Asp175) Pab Rb xHu|preincubated with irrelevant control peptide (left) and with Caspase 3, Cleaved (Asp175)|as Blocking Peptide (right).)

Product Categories/Family for CASP3, inhibitor

Molecular Biology; MB-Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 14790119
• NCBI GeneID: 836
• NCBI Accession #: NP_004337.2
• NCBI GenBank Nucleotide #: NM_004346.3
• UniProt Accession #: P42574; Q96AN1; Q96KP2; A8K5M2; D3DP53
• Molecular Weight: 31,608 Da
• NCBI Official Full Name: caspase-3 preproprotein
• NCBI Official Synonym Full Names: caspase 3, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP3
• NCBI Official Synonym Symbols: CPP32; SCA-1; CPP32B
• NCBI Protein Information: caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-3
• Protein Family: Caspase
• UniProt Gene Name: CASP3
• UniProt Synonym Gene Names: CPP32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Ref.8 Ref.15

Catalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

Enzyme regulation: Inhibited by isatin sulfonamides.

Subunit structure: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Ref.12

Subcellular location: Cytoplasm.

Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Post-translational modification: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Sequence similarities: Belongs to the peptidase C14A family.