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Sequence Information

Inhibin Beta E (INHbE) Recombinant Protein | INHbE recombinant protein

Recombinant Inhibin Beta E (INHbE)

Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Inhibin Beta E (INHbE); Recombinant Inhibin Beta E (INHbE); INHbE recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 95%
Form/Format
PBS, pH7.4, containing 0.05% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Applicable Applications for INHbE recombinant protein
Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Thr237~Ser350
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.2
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

Sequence Information

Sequence Information

Sequence Information

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 13.7kDa
Accurate Molecular Mass: 17/18kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
inhibin beta E chain
NCBI Official Synonym Full Names
inhibin, beta E
NCBI Official Symbol
INHBE
NCBI Protein Information
inhibin beta E chain; activin beta E; activin beta-E chain
UniProt Protein Name
Inhibin beta E chain
Protein Family
UniProt Gene Name
INHBE
UniProt Entry Name
INHBE_HUMAN

NCBI Description

INHBE is a member of the activin beta family (see INHBA; MIM 147290) that plays a role in pancreatic exocrine cell growth and proliferation (Hashimoto et al., 2006 [PubMed 16426570]).[supplied by OMIM, May 2008]

Uniprot Description

INHBE: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; regulation of MAPKKK cascade; cell development; growth

Research Articles on INHbE

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Product Notes

The INHbE inhbe (Catalog #AAA2012336) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Inhibin Beta E (INHbE) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Inhibin Beta E (INHbE) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the INHbE inhbe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inhibin Beta E (INHbE), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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