Rabbit anti-Human JUNB Polyclonal Antibody | anti-JUNB antibody

JUNB (Transcription Factor Jun-B) APC

Cat. Num. AAA6383389

• Gene Names: JUNB; AP-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JUNB; Polyclonal Antibody; JUNB (Transcription Factor Jun-B) APC; anti-JUNB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JUNB.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-JUNB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human JUNB, aa1-347 (NP_002220.1).
• Immunogen Sequence: MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGGVTEEQEGFADGFVKALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGAAVGTGSSYPTTTISYLPHAPPFAGGHPAQLGLGRGASTFKEEPQTVPEARSRDATPPVSPINMEDQERIKVERKRLRNRLAATKCRKRKLERIARLEDKVKTLKAENAGLSSTAGLLREQVAQLKQKVMTHVSNGCQLLLGVKGHAF
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of JUNB expression in transfected 293T cell line by JUNB polyclonal antibody. Lane 1: JUNB transfected lysate (35.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-JUNB antibody

The JunB transcription factor, also known as JunB proto-oncogene, JunB oncogene, transcription factor JunB, and transforming protein JunB, is a basic region leucine zipper DNA-binding protein, and a member of the Jun family of proteins, which also includes c-Jun and JunD. Jun proteins can homodimerize or heterodimerize with other Jun family members, or heterodimerize with Fos family members (cFos, FosB, Fra-1, and Fra-2) to form the activator protein 1 (AP-1) transcription factor. The various dimers differ in their ability to transactivate AP-1-dependent genes. Jun B8,9 and JunD1,10 are almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. JunB and C-Jun antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cyclin A is a direct transcriptional target of JunB driving cell proliferation JunB is an important regulator of erythroid differentiation, and is also important for the development of Th2 effector cells, normal Th2 cytokine expression pattern, and a complete Th2-dependent immune response in mice. JunB expression levels are increased in human colorectal adenocarcinoma specimens as compared to normal mucosa.

Product Categories/Family for anti-JUNB antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NM_002229.2
• UniProt Accession #: P17275; Q96GH3
• Molecular Weight: 35,879 Da
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: jun B proto-oncogene
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B; activator protein 1
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB
• UniProt Entry Name: JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; transcription coactivator activity; double-stranded DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to peptide hormone stimulus; response to light stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; cellular response to hormone stimulus; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; vasculogenesis; response to progesterone stimulus; response to corticosterone stimulus