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Typical Testing Data/Standard Curve (for reference only) (Mouse Activin A PicoKine ELISA Kit standard curve )

Mouse Activin A ELISA Kit | INHBA elisa kit

Mouse Activin A PicoKine ELISA Kit

Reactivity
Mouse
Synonyms
Activin A; Mouse Activin A PicoKine ELISA Kit; Inhibin beta A chain; inhibin; beta A; Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain; INHBA elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Natural and recombinant mouse Activin A
Sequence Length
424
Samples
Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Activin A PicoKine ELISA Kit standard curve )

Typical Testing Data/Standard Curve (for reference only) (Mouse Activin A PicoKine ELISA Kit standard curve )
Related Product Information for INHBA elisa kit
Principle of the assay: mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Activin A amount of sample captured in plate.
Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factorbeta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins andactivins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
47,392 Da
NCBI Official Full Name
Inhibin beta A chain
NCBI Official Synonym Full Names
inhibin beta-A
NCBI Official Symbol
Inhba
NCBI Protein Information
inhibin beta A chain; activin beta-A chain
UniProt Protein Name
Inhibin beta A chain
Protein Family
UniProt Gene Name
Inhba
UniProt Entry Name
INHBA_MOUSE

Uniprot Description

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region

Molecular Function: identical protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; palate development; negative regulation of cell cycle; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; ovarian follicle development; hair follicle development; cell cycle arrest; hemoglobin biosynthetic process; response to drug; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; negative regulation of hair follicle development; progesterone secretion; regulation of transcription from RNA polymerase II promoter; mesoderm formation; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; cell development; G1/S transition of mitotic cell cycle; growth

Research Articles on INHBA

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Product Notes

The Mouse INHBA inhba (Catalog #AAA175817) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175817 ELISA Kit recognizes Mouse INHBA. It is sometimes possible for the material contained within the vial of "Activin A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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