Cholera Toxin, B Subunit Infectious Disease
Cholera Toxin, B Subunit (Choleragenoid)
Purity
Molecular Biology GradeMigrates as a single major band in slab gel electrophoresis under non-denaturing conditions.
Synonyms
Cholera Toxin; B Subunit; B Subunit (Choleragenoid); B Subunit infectious disease
Purity/Purification
Molecular Biology Grade
Migrates as a single major band in slab gel electrophoresis under non-denaturing conditions.
Migrates as a single major band in slab gel electrophoresis under non-denaturing conditions.
Form/Format
Supplied as a lyophilized powder in 0.05M Tris, 0.2M sodium chloride, 0.001M EDTA•Na2, pH 7.5, 0.003M sodium azide. Cholera toxin and native subunits all undergo treatment for the removal of contaminating endotoxin and are sterile as packaged.
Activity
Exhibits comparable ganglioside binding activity in a hemagglutination assay,
when compared to a standard solution of B subunit at the same protein concentration.
when compared to a standard solution of B subunit at the same protein concentration.
Contaminants
Transferase activity is nearly zero when compared to an equivalent amount of holotoxin in an ADP-ribosyltransferase assay.
Note
Cholera toxin and native subunits all undergo treatment for the removal of contaminating endotoxin and are sterile as packaged.
Reconstitution
Reconstitute with 100ul sterile dH2O
Preparation and Storage
Lyophilized powder stoppered under vacuum. It is recommended that this material be stored at 4 degree C prior to and following reconstitution. DO NOT FREEZE.
Related Product Information for Cholera Toxin, B Subunit infectious disease
Cholera toxin is an oligomeric protein of MW 84kD and consists of a single A subunit surrounded by five B subunits. It is a potent activator of adenylate cyclase and is the pathogenic agent responsible for the symptoms of cholera. The B subunit (choleragenoid) is responsible for the binding of the holotoxin to G M1 ganglioside receptors on mammalian cell surfaces and facilitates entrance of the A subunit into the cell. The A subunit bears the ADP-ribosyl-transferase activity, which deregulates the G sprotein causing activation of adenylate cyclase. Due to the ubiquitous occurrence of the GM1 ganglioside receptor on eukaryotic cell membranes, cholera toxin activates adenylate cyclase in a wide variety of systems.
Cholera toxin has become a powerful research tool not only in microbiology, but in the fields of physiology, cell biology and biochemistry, as well. Because of the effect on adenylate cyclase, cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms. In addition, cholera toxin acts as an adjuvant through the stimulation of B-lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
Cholera toxin is isolated from Vibrio cholerae type Inaba 569B by modification of the methods of Rappaport et al. and Mekalanos et al. When equal weights are compared, the A subunit exhibits 3 to 5 times the transferase activity of the holotoxin. The B subunit exhibits little to no transferase activity.
Cholera toxin has become a powerful research tool not only in microbiology, but in the fields of physiology, cell biology and biochemistry, as well. Because of the effect on adenylate cyclase, cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms. In addition, cholera toxin acts as an adjuvant through the stimulation of B-lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
Cholera toxin is isolated from Vibrio cholerae type Inaba 569B by modification of the methods of Rappaport et al. and Mekalanos et al. When equal weights are compared, the A subunit exhibits 3 to 5 times the transferase activity of the holotoxin. The B subunit exhibits little to no transferase activity.
Product Categories/Family for Cholera Toxin, B Subunit infectious disease
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Product Notes
The Cholera Toxin, B Subunit (Catalog #AAA655235) is an Infectious Disease and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cholera Toxin, B Subunit, Infectious Disease" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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