Mouse ACHE Monoclonal Antibody | anti-ACHE antibody

ACHE Antibody (C-term)

Cat. Num. AAA9232068

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Human, Mouse, Rat, Hamster
• Applications: Western Blot, ELISA
• Synonyms: ACHE; Monoclonal Antibody; ACHE Antibody (C-term); Acetylcholinesterase; AChE; anti-ACHE antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This ACHE antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 597-631 amino acids from the C-terminal region of human ACHE.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 614

• Applicable Applications for anti-ACHE antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Source: Human
• Antigen Region: 597-631
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-ACHE Antibody (C-term) at 1:2000 dilutionLane 1: COS-7 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: NIH/3T3 whole cell lysatesLane 5: rat cerebellum lysatesLane 6: Raji whole cell lysatesLane 7: CHO whole cell lysates Lysates/proteins at 20 ug per lane. SecondaryGoat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-ACHE Antibody (C-term) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateLane 3: COS-7 whole cell lysateLane 4: NIH/3T3 whole cell lysateLane 5: mouse cerebellum lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ACHE antibody

Description: Purified Mouse Monoclonal Antibody (Mab)

Background: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

Product Categories/Family for anti-ACHE antibody

Neuroscience

References

Soreq H.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:9688-9692(1990). Karpel R.,et al.Exp. Cell Res. 210:268-277(1994). Yang L.,et al.Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4557239
• NCBI GeneID: 43
• NCBI Accession #: NP_000656.1
• NCBI GenBank Nucleotide #: NP_000656.1
• UniProt Accession #: P22303
• Molecular Weight: 67796
• NCBI Official Full Name: acetylcholinesterase isoform E4-E6
• NCBI Official Synonym Full Names: acetylcholinesterase (Cartwright blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Achelase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Membrane protein, GPI anchor; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; synapse; basal lamina; neuromuscular junction; nucleus; cell junction

Molecular Function: collagen binding; serine hydrolase activity; protein binding; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: negative regulation of synaptic transmission, cholinergic; nervous system development; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; synaptic transmission; cell proliferation; synaptogenesis; positive regulation of protein secretion; amyloid precursor protein metabolic process; retina development in camera-type eye; phospholipid metabolic process; neurotransmitter receptor biosynthetic process; receptor internalization; response to wounding; phosphatidylcholine biosynthetic process; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen