Human Inhibin betaA ELISA Kit | INB-betaA elisa kit

Human Inhibin betaA ELISA Kit

Cat. Num. AAA733369

• Gene Names: INHBA; EDF; FRP
• Reactivity: Human
• Synonyms: Inhibin betaA; Human Inhibin betaA ELISA Kit; Inhibin bA ELISA Kit; Inhibin βA ELISA Kit; Inhibin betaA ELISA Kit (Human); INB-betaA elisa kit

• Reactivity: Human

• Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INB-betaA elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This LEFTY1 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of LEFTY1 in the sample, this LEFTY1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus LEFTY1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of LEFTY1 utilizes a multiclonal anti-LEFTY1 antibody and an LEFTY1-HRP conjugate. The assay sample and buffer are incubated together with LEFTY1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LEFTY1 concentration since LEFTY1 from samples and LEFTY1-HRP conjugate compete for the anti-LEFTY1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LEFTY1 from the sample, fewer sites are left to bind LEFTY1-HRP conjugate. Standards of known LEFTY1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of LEFTY1. The LEFTY1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for INB-betaA elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 119614547
• NCBI GeneID: 3624
• UniProt Accession #: P08476; Q14599
• Molecular Weight: 47,442 Da
• NCBI Official Full Name: inhibin, beta A (activin A, activin AB alpha polypeptide), isoform CRA_a
• NCBI Official Synonym Full Names: inhibin, beta A
• NCBI Official Symbol: INHBA
• NCBI Official Synonym Symbols: EDF; FRP
• NCBI Protein Information: inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)
• UniProt Protein Name: Inhibin beta A chain
• UniProt Gene Name: INHBA
• UniProt Synonym Gene Names: EDF
• UniProt Entry Name: INHBA_HUMAN

NCBI Description

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; ovarian follicle development; cell-cell signaling; hair follicle development; erythrocyte differentiation; cell cycle arrest; cell differentiation; hemoglobin biosynthetic process; negative regulation of B cell differentiation; response to drug; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; growth; G1/S transition of mitotic cell cycle