Typical Testing Data/Standard Curve (for reference only)
Mouse Prestin ELISA Kit | Slc26a5 elisa kit
Mouse Prestin ELISA Kit
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 33% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PRES ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRES antibody and an PRES-HRP conjugate. The assay sample and buffer are incubated together with PRES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRES concentration since PRES from samples and PRES-HRP conjugate compete for the anti-PRES antibody binding site. Since the number of sites is limited, as more sites are occupied by PRES from the sample, fewer sites are left to bind PRES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRES concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
molecular motor protein; involved in the electromechanical properties of hearing [RGD, Feb 2006]
Uniprot Description
Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site ().
Research Articles on Slc26a5
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Product Notes
The Mouse Slc26a5 slc26a5 (Catalog #AAA7256311) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7256311 ELISA Kit recognizes Mouse Slc26a5. It is sometimes possible for the material contained within the vial of "Prestin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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