Mouse Activin A ELISA Kit | ACA-A elisa kit

Mouse Activin A ELISA Kit

Cat. Num. AAA723197

• Gene Names: INHBA; EDF; FRP
• Reactivity: Mouse
• Synonyms: Activin A; Mouse Activin A ELISA Kit; ACA-A elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This ACTIVIN-A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ACTIVIN-A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACA-A elisa kit

Principle of the Assay: ACTIVIN-A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTIVIN-A antibody and an ACTIVIN-A-HRP conjugate. The assay sample and buffer are incubated together with ACTIVIN-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTIVIN-A concentration since ACTIVIN-A from samples and ACTIVIN-A-HRP conjugate compete for the anti-ACTIVIN-A antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTIVIN-A from the sample, fewer sites are left to bind ACTIVIN-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTIVIN-A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ACA-A elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119614547
• NCBI GeneID: 3624
• Molecular Weight: 47,442 Da
• NCBI Official Full Name: inhibin, beta A (activin A, activin AB alpha polypeptide), isoform CRA_a
• NCBI Official Synonym Full Names: inhibin, beta A
• NCBI Official Symbol: INHBA
• NCBI Official Synonym Symbols: EDF; FRP
• NCBI Protein Information: inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)
• UniProt Protein Name: Inhibin beta A chain
• UniProt Gene Name: INHBA
• UniProt Synonym Gene Names: EDF
• UniProt Entry Name: INHBA_HUMAN

NCBI Description

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; hormone activity; peptide hormone binding; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; cell-cell signaling; hair follicle development; ovarian follicle development; erythrocyte differentiation; cell cycle arrest; cell differentiation; response to drug; negative regulation of B cell differentiation; hemoglobin biosynthetic process; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; positive regulation of follicle-stimulating hormone secretion; negative regulation of cell growth; cell development; growth; G1/S transition of mitotic cell cycle