Activating Transcription Factor 2, Control Cell Extracts Immunoglobulin
Activating Transcription Factor 2, Control Cell Extracts (ATF2)
Purity
Lysate
Synonyms
Activating Transcription Factor 2; Control Cell Extracts; Control Cell Extracts (ATF2); Control Cell Extracts immunoglobulin
Purity/Purification
Lysate
Form/Format
Supplied as a liquid in 62.5mM Tris-HCl, pH 6.8, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue or phenol red.
Preparation and Storage
-20 degree C
Related Product Information for Activating Transcription Factor 2, Control Cell Extracts immunoglobulin
Total cell extracts from NIH/3T3 cells prepared without treatment, suitable for use as a negative control.
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (2-4). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (2-4). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
Product Categories/Family for Activating Transcription Factor 2, Control Cell Extracts immunoglobulin
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Product Notes
The Activating Transcription Factor 2, Control Cell Extracts (Catalog #AAA652633) is an Immunoglobulin and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Activating Transcription Factor 2, Control Cell Extracts, Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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