Testing Data
Immobilized Trypsin, Silica Recombinant Protein
Immobilized Trypsin, Silica
Synonyms
Immobilized Trypsin; Silica; Trypsinogen; Silica recombinant protein
Host
Yeast
Tag
TAG-Free
Species
Swine
Enzyme Commission Number
EC 3.4.21.4
Matrix
cross-linked silica
Average Particle Size
200um
Ligand Density
10 mg protein/ml matrix
Maximum Flow Rate, Pressure
300cm/h, 3 bar
Digestion Condition
Catalytic pHRange: 8.0 ~10.0
Catalytic Temperature Range: 20 ~ 37 degree C
Enzyme: Substrate Ratio: 1:200 ~ 1:3,000
Catalytic Temperature Range: 20 ~ 37 degree C
Enzyme: Substrate Ratio: 1:200 ~ 1:3,000
Preparation and Storage
Store product at 2 -4 degree C for up to 6 months.
Testing Data
Testing Data
Related Product Information for Immobilized Trypsin, Silica recombinant protein
Recombinant tag-free swine trypsin (10-end) was expressed in yeast cells.
Silica immobilized proteases are amenable to large-scale proteolysis processes. Trypsin is a serine protease that is produced in the acinar exocrine cells of the pancreas. The enzyme cleaves peptides at the C-terminal side of lysine and arginine amino acid residues. Recombinant pancreatic trypsin is a widely biochemical tool used in processes, which include: recombinant insulin production, cell culture, cell fermentation, protein peptide mapping, proteomic sequencing and cell dissociation. Trypsin function is inhibited by serine protease inhibitors (e.g. TLCK, PMSF), and metal chelating agents (e.g. EDTA).
Immobilized trypsin is covalently cross-linked to a silica matrix circumventing the need for enzyme removal after cleavage. The resin can be reused ~20 times after regenerating when proper storage conditions are followed.
Silica immobilized proteases are amenable to large-scale proteolysis processes. Trypsin is a serine protease that is produced in the acinar exocrine cells of the pancreas. The enzyme cleaves peptides at the C-terminal side of lysine and arginine amino acid residues. Recombinant pancreatic trypsin is a widely biochemical tool used in processes, which include: recombinant insulin production, cell culture, cell fermentation, protein peptide mapping, proteomic sequencing and cell dissociation. Trypsin function is inhibited by serine protease inhibitors (e.g. TLCK, PMSF), and metal chelating agents (e.g. EDTA).
Immobilized trypsin is covalently cross-linked to a silica matrix circumventing the need for enzyme removal after cleavage. The resin can be reused ~20 times after regenerating when proper storage conditions are followed.
Product Categories/Family for Immobilized Trypsin, Silica recombinant protein
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Product Notes
The Immobilized Trypsin, Silica (Catalog #AAA517722) is a Recombinant Protein produced from Yeast and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Immobilized Trypsin, Silica, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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