Rabbit MIA3 Polyclonal Antibody | anti-MIA3 antibody

MIA3 Antibody

Cat. Num. AAA9609939

• Gene Names: MIA3; ARNT; D320; TANGO; TANGO1; UNQ6077
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MIA3; Polyclonal Antibody; MIA3 Antibody; ARNT; C219 reactive peptide; C219-reactive peptide; D320; Melanoma inhibitory activity family; member 3; Melanoma inhibitory activity protein 3; Mia3; MIA3_HUMAN; RP11-378J18.4; TANGO; Transport and Golgi organization protein 1; UNQ6077; anti-MIA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MIA3 antibody detects endogenous levels of total MIA3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 785

• Applicable Applications for anti-MIA3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human MIA3
• Subcellular Location: Endoplasmic Reticulum Membrane. Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane.
• Tissue Specificity: Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.
• Predicted Cross Reactivity: Dog, Chicken
• Similarity: Dog (83%), Chicken (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of MIA3 using Jurkat whole cell lysates)

Related Product Information for anti-MIA3 antibody

Function: Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255).
Subunit Structure: Interacts with CTAGE5 (PubMed:21525241). Interacts (via SH3 domain) with COL7A1 (PubMed:19269366). Interacts with the COPII coat subunits SEC23A, SEC23B and maybe SEC24C (PubMed:19269366, PubMed:27551091). May interact with APOB and MIA2 (PubMed:27138255).
Similarity: The proline-rich domain (PRD) contains repeated PPP motifs. A single PPP motif is necessary and sufficient to mediate interaction with the COPII coat subunits SEC23A and SEC23B.Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region. Belongs to the MIA/OTOR family. Tango1 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 664806007
• NCBI GeneID: 375056
• NCBI Accession #: NP_001287796.1
• NCBI GenBank Nucleotide #: NM_001300867.1
• UniProt Accession #: Q5JRA6; Q14083; Q3S4X3; Q5JRA5; Q5JRB0; Q5JRB1; Q5JRB2; Q6UVY8; A8K2S0; A8MT05; A8MT13; B7Z430
• Molecular Weight: Observed: 214 kDa; Predicted: 214 kDa
• NCBI Official Full Name: transport and Golgi organization protein 1 homolog isoform 2
• NCBI Official Synonym Full Names: MIA SH3 domain ER export factor 3
• NCBI Official Symbol: MIA3
• NCBI Official Synonym Symbols: ARNT; D320; TANGO; TANGO1; UNQ6077
• NCBI Protein Information: transport and Golgi organization protein 1 homolog
• UniProt Protein Name: Transport and Golgi organization protein 1 homolog
• Protein Family: Melanoma inhibitory activity protein
• UniProt Gene Name: MIA3
• UniProt Synonym Gene Names: KIAA0268ImportedManual assertion inferred from database entriesiEMBL:BAA13448.1; TANGO1 PublicationManual assertion based on opinion iniRef.4; TANGO1

Uniprot Description

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255).