Testing Data
Immobilized Lys-c, Silica Recombinant Protein | Lys-c recombinant protein
Immobilized Lys-c, Silica
Synonyms
Immobilized Lys-c; Silica; API; Protease I; Lysyl endopeptidase; Lys-c recombinant protein
Host
E Coli
Tag
TAG-Free
Species
Bacteria
Enzyme Commission Number
EC 3.4.21.50
Matrix
cross-linked silica
Average Particle Size
200 um
Ligand Density
5 mg protein/ml matrix
Maximum Flow Rate, Pressure
300cm/h, 2 bar
Digestion Condition
Catalytic pHRange: 8.0 ~ 9.5
Catalytic Temperature Range: 15 ~ 37 degree C
Enzyme: Substrate Ratio: 1:200 ~ 1:2,000
Catalytic Temperature Range: 15 ~ 37 degree C
Enzyme: Substrate Ratio: 1:200 ~ 1:2,000
Preparation and Storage
Store product at 2 -4 degree C for up to 6 months.
Testing Data
Testing Data
Related Product Information for Lys-c recombinant protein
Recombinant tag-free Lys-c (Lysyl-Endopeptidase) was expressed in E Coli cells.
Silica immobilized proteases are amenable to large-scale proteolysis processes. Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 -37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.
Immobilized Lysyl-Endopeptidase is covalently cross-linked to a silica matrix circumventing the need for enzyme removal after cleavage. The resin can be reused ~20 times after regenerating when proper storage conditions are followed.
Silica immobilized proteases are amenable to large-scale proteolysis processes. Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 -37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.
Immobilized Lysyl-Endopeptidase is covalently cross-linked to a silica matrix circumventing the need for enzyme removal after cleavage. The resin can be reused ~20 times after regenerating when proper storage conditions are followed.
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Product Notes
The Lys-c (Catalog #AAA517172) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Immobilized Lys-c, Silica, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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