CD127 recombinant protein

Recombinant Mouse CD127 Protein

Cat. Num. AAA8305152

• Gene Names: Il7r; CD127; IL-7Ralpha
• Applications: ELISA, Western Blot, SDS-Page
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: CD127; Recombinant Mouse CD127 Protein; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD127 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: ESGNAQDGDL EDADADDHSF WCHSQLEVDG SQHLLTCAFN DSDINTANLE FQICGALLRV KCLTLNKLQD IYFIKTSEFL LIGSSNICVK LGQKNLTCKN MAINTIVKAE APSDLKVVYR KEANDFLVTF NAPHLKKKYL KKVKHDVAYR PARGESNWTH VSLFHTRTTI PQRKLRPKAM YEIKVRSIPH NDYFKGFWSE WSPSSTFETP EPKNQGGWDV DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
• Sequence Length: 459

• Applicable Applications for CD127 recombinant protein: ELISA (EIA), Western Blot (WB), SDS-PAGE
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CD127 recombinant protein

Recombinant Mouse CD127 Protein is produced by mammalian expression system and the target gene encoding Glu21-Asp239 is expressed with a Fc tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 341940827
• NCBI GeneID: 16197
• UniProt Accession #: P16872
• NCBI Official Full Name: Interleukin-7 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 7 receptor
• NCBI Official Symbol: Il7r
• NCBI Official Synonym Symbols: CD127; IL-7Ralpha
• NCBI Protein Information: interleukin-7 receptor subunit alpha
• UniProt Protein Name: Interleukin-7 receptor subunit alpha
• UniProt Gene Name: Il7r
• UniProt Synonym Gene Names: IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA
• UniProt Entry Name: IL7RA_MOUSE

NCBI Description

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

IL7R: Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell- positive/NK-cell-positive (T(-)B(+)NK(+) SCID). A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS. Belongs to the type I cytokine receptor family. Type 4 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity

Biological Process: positive regulation of T cell differentiation in the thymus; B cell proliferation; negative regulation of T cell mediated cytotoxicity; homeostasis of number of cells; cell morphogenesis; immunoglobulin production; cell growth; lymph node development; regulation of cell size; T cell differentiation