IL-7 Recombinant Protein | IL7 recombinant protein

RECOMBINANT HUMAN INTERLEUKIN-7

Cat. Num. AAA232250

• Gene Names: IL7; IL-7
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98 % by SDS PAGE and HPLC
• Synonyms: IL-7; RECOMBINANT HUMAN INTERLEUKIN-7; IL7 recombinant protein

• Purity/Purification: >98 % by SDS PAGE and HPLC
• Form/Format: Purified recombinant protein expressed in E Coli - lyophilised
• Concentration: Total protein concentration 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 177

• Applicable Applications for IL7 recombinant protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant human IL-7 may be used as a standard for ELISA applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).; Western Blot: Recombinant human IL-7 may be used as the positive control for western blotting applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4 ng/well; Functional Assays: Minimum Dilution: 0.1ng/ml; Maximum Dilution: 10ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0 ng/lane
• Reconstitution: Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Buffer Solution: 10mM Acetic Acid
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for IL7 recombinant protein

Recombinant human IL-7 is 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence. IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg. Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clones BVD10-40F6 and BVD10-11C10 in ELISA.

NCBI and Uniprot Product Information

• NCBI GI #: 4504677
• NCBI GeneID: 3574
• NCBI Accession #: NP_000871.1
• NCBI GenBank Nucleotide #: NM_000880.3
• UniProt Accession #: P13232; Q5FBY5; Q5FBY9; A0N0L3
• Molecular Weight: Protein Molecular Weight: 17.4KD (153 amino acids)
• NCBI Official Full Name: interleukin-7 isoform 1
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Synonym Gene Names: IL-7
• UniProt Entry Name: IL7_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Uniprot Description

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cytokine; Apoptosis; Cell development/differentiation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of organ growth; humoral immune response; organ morphogenesis; T cell lineage commitment; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; positive regulation of cell proliferation; negative regulation of catalytic activity; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption