Interleukin-7 Recombinant Protein | IL-7 recombinant protein

Human Interleukin-7 Recombinant

Cat. Num. AAA553294

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-7; Human Interleukin-7 Recombinant; il-7; il7; IL-7 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-7 was lyophilized from a 0.2 um filtered PBS solution pH 4.5.
• Sequence: MFHVSFRYIF GLPPLILVLL PVASSDCDIE GKDGKQYESV LMVSIDQLLD SMKEIGSNCL NNEFNFFKRH ICDANKEGMF LFRAARKLRQ FLKMNSTGDF DLHLLKVSEG TTILLNCTGQ VKGRKPAALG EAQPTKSLEE NKSLKEQKKL NDLCFLKRLL QEIKTCWNKI LMGTKEH
• Sequence Length: 177

• Domain: Interleukin-7/9
• Host Note: Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-7 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 17 kDa.; ; ; Recombinant IL-7 is a disulfide-linked monomeric protein consisting of 153 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-7 recombinant protein

IL7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL7.Human (152 amino acids; 17.4 kDa) and murine IL7 (129 amino acids) show 60 % sequence homology at the protein level. The human IL7 receptor is an integral strongly glycosylated membrane proteins of 76 kDa expressed on activated T-cells. This receptor has been designated as CD127. IL7 receptors are expressed on pre-B-cells and their progenitors. They are not expressed on mature B-cells. IL7 receptors are expressed also on bone marrow macrophages. Functional IL7 receptors are found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B- cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line

Product Categories/Family for IL-7 recombinant protein

Cytokines

References

• tslp and IL-7 use two different mechanisms to regulate human cd4+ t cell homeostasis .J. Exp. Med., Sep 2009; 206: 2111 - 2119.
• self-peptides prolong survival in murine autoimmunity via reduced il-2/IL-7-mediated stat5 signaling, cd8 coreceptor, and v2 down-regulation .J. Immunol., Sep 2009; 183: 3130 - 3138.
• rap signaling is crucial for the competence of IL-7 response and the development of b-lineage cells .Blood, Aug 2009; 114: 1768 - 1775.
• cutting edge: IL-7 regulates the peripheral pool of adult ror+ lymphoid tissue inducer cells .J. Immunol., Aug 2009; 183: 2217 - 2221.
• effect of end-stage renal disease on b-lymphocyte subpopulations, IL-7, baff and baff receptor expression .Nephrol. Dial. Transplant., Aug 2009; 10.1093/ndt/gfp397.