Rabbit SOX10 Polyclonal Antibody | anti-SOX10 antibody

SOX10 Antibody

Cat. Num. AAA154448

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human, Mouse, Rat; Predicted: Pig (100%), Chicken (89%), Goat (100%)
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: SOX10; Polyclonal Antibody; SOX10 Antibody; SRY (sex determining region Y)-box 10; DOM;WS4; PCWH; WS2E; WS4C; SRY-box 10; anti-SOX10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig (100%), Chicken (89%), Goat (100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SOX10 Antibody is predicted to not cross-react with other SOX familiy members.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 466

• Applicable Applications for anti-SOX10 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SOX10 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human SOX10.
• Buffer: SOX10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles; Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SOX10 in rat heart tissue lysate with SOX10 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SOX10 in rat heart tissue with SOX10 Antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SOX10 in rat heart tissue with SOX10 Antibody at 20 μg/mL.)

Related Product Information for anti-SOX10 antibody

SOX10 Antibody: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of the cell fate (1). The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins (2). This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development (2). Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschprung disease (3). SLC30A8 expression in beta-cells was found to be influenced by cytokine expression, particularly IFN-gamma and IL-1beta (4).

Product Categories/Family for anti-SOX10 antibody

Signal Transduction; Stem Cell

References

1. Pusch C, Hustert E, Pfeifer D, et al. The SOX10/Sox10 gene from human and mouse: sequence, expression, and transactivation by the encoded HMG domain transcription factor. Hum. Genet. 1998; 103:115-23. 2. Rehberg S, Lischka P, Glaser G, et al. Sox10 is an active nucleocytoplasmic shuttle protein, and shuttling is crucial for Sox10-mediated transactivation. Mol. Cell Biol. 2002; 22:5826-34. 3. Pingault V, Bondurand N, Kuhlbrodt K, et al. SOX10 mutations in patients with Waardenburg-Hirschprung disease. Nat. Genet. 1998; 18:171-3.

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872
• NCBI GenBank Nucleotide #: NM_006941.3
• UniProt Accession #: P56693; Q6FHW7; B4DV62
• Molecular Weight: Predicted: 51 kDa; Observed: 54 kDa
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• Protein Family: Transcription factor
• UniProt Gene Name: SOX10

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; transcription coactivator activity

Biological Process: anatomical structure morphogenesis; regulation of transcription from RNA polymerase II promoter

Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c