Human Interleukin-4 receptor ELISA Kit | IL4R elisa kit

Human Interleukin-4 receptor ELISA Kit

Cat. Num. AAA763281

• Gene Names: IL4R; CD124; IL4RA; IL-4RA
• Reactivity: Human
• Synonyms: Interleukin-4 receptor; Human Interleukin-4 receptor ELISA Kit; IL4R (Interleukin-4 receptor subunit alpha)/IL-4 receptor subunit alpha/IL-4R subunit alpha/IL-4R-alpha/IL-4RA/IL4RA/interleukin 4 receptor/interleukin-4 receptor alpha chain; IL4R elisa kit

• Reactivity: Human
• Sequence Length: 825

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IL4R elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human IL4R antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IL4R antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL4R amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman IL4R can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 33834
• NCBI GeneID: 3566
• UniProt Accession #: P24394; Q96P01; Q9H181; Q9H182; Q9H183; Q9H184; Q9H185; Q9H186; Q9H187; B4E076; B9EKU8; H3BSY5
• Molecular Weight: 88,267 Da
• NCBI Official Full Name: interleukin 4 receptor
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: IL4R
• NCBI Official Synonym Symbols: CD124; IL4RA; IL-4RA
• NCBI Protein Information: interleukin-4 receptor subunit alpha
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• Protein Family: Interleukin-4 receptor
• UniProt Gene Name: IL4R
• UniProt Synonym Gene Names: IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP
• UniProt Entry Name: IL4RA_HUMAN

NCBI Description

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 16p12.1-p11.2

Cellular Component: extracellular space; integral to plasma membrane; intracellular; receptor complex

Molecular Function: interleukin-4 receptor activity; protein binding; receptor signaling protein activity

Biological Process: defense response to protozoan; immune response; immunoglobulin mediated immune response; negative regulation of T-helper 1 cell differentiation; ovulation; positive regulation of immunoglobulin production; positive regulation of macrophage activation; positive regulation of mast cell degranulation; positive regulation of T-helper 2 cell differentiation; production of molecular mediator of acute inflammatory response; regulation of cell proliferation; response to estrogen stimulus; signal transduction

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To; Ige Responsiveness, Atopic